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The methylation process is responsible for repairing and regenerating cells, detoxification, neurotransmitter production which influence moods and sleep, controlling homocysteine, appropriate stress response, regulating inflammation, aiding the liver in processing fats, antioxidant production, and healthy immune system function

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HepG2 cells were seeded onto a cell slide in a 24-well plate and transfected with plasmid vectors according to the transfection reagent instructions