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Preparation Condition 3 (EDTA → −80 o C → deproteinization): Deproteinization may not be necessary to prevent spontaneous oxidation of free GSH Unexpectedly, we found no difference in the levels of total GSH, free GSH, GSSG, or the ratio of free GSH to GSSG between the EDTA-treated plasma samples which were stored at −80 o C (Preparation Condition 2 and Preparation Condition 3)