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5A-1MQ-treated mice switched from WD to LD (WD/LD-T) showed a rather reduced relative abundance of Akkermansia compared to vehicle-treated DIO animals switched from WD to LD (WD/LD-V), suggesting a unique microbiome mechanism associated with weight loss in the vehicle-treated group switched from WD to LD compared to the 5A-1MQ-treated group that underwent the same diet switch

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It is studied in preclinical research across multiple cellular signalling contexts, including extracellular matrix remodelling, fibroblast activity models, copper transport and chaperone studies, and broad gene expression modulation assays