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For tumour xenograft models, 1 × 10 6 HCC-1806 cells or 5 × 10 6 PC3 cells were injected into the right fourth mammary fat pad or hind flank of athymic nude NU/J mice (The Jackson Laboratory, 002019) in 100 µl sterile PBS or a 1:1 mixture of cell suspension in PBS and Matrigel (Corning, 356237) using a 29-gauge 1 ml insulin syringe