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Rodent model Attenuated hepatic steatosis in obese mice In the same 30-day study, high-dose animals showed a 73% reduction in microvesicular steatosis score against controls, lower terminal liver weight after adjustment for body weight, reduced liver triglycerides and macrophage infiltration, and normalised alanine transaminase, aspartate transaminase and ketone body levels
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After incubation for 15 min at 30 °C, 1,000 rpm using a 3 mm shaking diameter, cell debris was removed by centrifugation (4,400 g at 4 °C for 30 min) and the supernatants were analysed by HPLC–MS at two different dilutions (undiluted and four times diluted with 67% methanol, 0.2% hydrochloric acid in H 2 O)
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